Cambridge Isotope Laboratories, Inc’s Post

https://lnkd.in/ekTvMA8G Did you know that FLC assays are one of the most sensitive assays for MGRS management? Join our Plasma Proteins Science Day on November 9 and learn about free light chains and other advances in protein testing. #PPSD23

This amazing article precisely describes the interaction of 17 antibiotics with the E.coli 70S ribosome at atomic resolution! The #cryoEM structures of the ribosomal RNA-bound antibiotics together with molecular modeling and MD simulations represent the first high-precision atlas of several antibiotic families interacting with a conserved water- and ion-mediated network. These detailed structural insights will serve as the basis for structure-based #DrugDesign of new antibiotic derivatives in future. #SBDD

📢 Expand your knowledge on target protein degradation studies. Don’t miss our June 27 webinar with Aman Makaju from Amgen, who will share high-throughput proteomic approaches to better understand protein-protein interactions and target protein degradation induced by prospective molecular glues Register to get into 🔔 🔴 The use of mass spectrometry to perform Targeted Protein Degradation (TPD) studies. 🔴Use of high-throughput proteomic approaches to better understand protein-protein interactions and target protein degradation induced by prospective molecular glues. 🔴Learn about the novel Orbitrap Astral Mass Spectrometer and it's capabilities. 🔔 Register here https://lnkd.in/dA23dtg9 #thermofisherscientific #orbitrap #orbitrapastral #astral

Among the omics approaches, proteomics plays a critical role in monitoring protein expression, interactions and modifications. However, manually handling samples tends to be time-consuming and a significant source of variability. But it doesn’t have to be that way. Let us help you untangle the complexities of proteomics. Get started today: https://becls.co/40gvQwb #Proteomics #UntangleTheComplexities #LabAutomation

This new preprint from Paul Kremer, Elizabeth Lampros, Allison Blocker and Adam Barb at the The University of Georgia shows the importance of the glycoform on N162 of FcgRIIIa and how this impacts binding to the IgG Fc domain and resulting ADCC. In previous work they showed a high degree glycan heterogeneity at this site in healthy donors resulting in high and low-affinity variants of the Fc receptor. Now they explore the mechanism underlying this. DOI: https://lnkd.in/eQyy8q8Q Be careful when purchasing Fc receptors. There are many suppliers using many different expression platforms. Glyosylation of Fc gamma receptors is critical to their activity so at the very least please ensure any receptors you purchase have been produced in a mammalian cell line. See gammaproteins.com for our mammalian expressed human FcγRs.

Detecting nucleic acids is faster and easier than ever thanks to isothermal amplification methods. Adding CRISPR-based detection to these assays can further improve these methods for research and diagnostics. In The Scientist’s Journal Club, Kuangwen Hsieh will present his recent work comparing various single-step molecular detection methods that combine isothermal amplification with CRISPR. 📅 December 11th, 2023 @ 2:30PM ET Register for the webinar now to join the discussion: https://ow.ly/GrPV50Q6oFe

🔬 Breaking boundaries in #NASH research! Exciting strides are being made towards an effective #pharmacotherapy for NASH, the result of dedicated investigations into its molecular complexities. Read more 👉 https://ow.ly/Uqeh50Q0TOG #NAFLD #MASH #MASLD #Fattyliver

A nice example of why glycosylation matters! As antibody scientists we know that glycosylation of the antibody Fc domain can have a major impact on binding to FcgRs and resulting ADCC/ADCP but we tend to easily forget that the reverse is also true. In this paper they very nicely demonstrate the importance of glycosylation of N162 on FcgRIIIa (CD16a) and how different glycoforms can have a major impact on binding to the Fc domain and ADCC. Patients have been shown to have significant glycoform heterogeneity at this site on FcgRIIIa so this should be an important consideration in the clinic. Your perfectly engineered antibody might not be so potent if your patients have an abundance of the low-affinity glycoform of FcgRIIIa. As an aside, this is also an important consideration when purchasing Fc receptors. Not long ago we were running FcgRIIIa chromatography columns for some work that will be published very soon. We got some very odd results for our IgG4s which it turned out was due to the fact that the only commercially available FcgRIIIa columns use non-glycosylated receptor produced in prokaryotes. Link to the preprint in the original post from Gamma Proteins.

Their are plenty of reasons to trust Bio X Cell's #InVivoPlus antibody range for your experiments! 🔬 > 95% purity and integrity confirmed with SDS page Ultra-low endotoxin levels (< 1.0 EU/mg) Aggregation levels of ≤ 5% Advanced activity validation via flow cytometry, ELISA, and/or Immunoblot Screened extensively for murine pathogens Free of preservatives, stabiliser and carrier proteins Mentioned in thousands of scientific publications Want to learn more about how this range can level up your research? 👇 https://lnkd.in/e7gifuVz #invivoantibody #lifescience #bioxcell